import mathutils import math # create a location matrix mat_loc = mathutils.Matrix.Translation((2.0, 3.0, 4.0)) # create an identitiy matrix mat_sca = mathutils.Matrix.Scale(0.5, 4, (0.0, 0.0, 1.0)) # create a rotation matrix mat_rot = mathutils.Matrix.Rotation(math.radians(45.0), 4, 'X') # combine transformations mat_out = mat_loc * mat_rot * mat_sca print(mat_out) # extract components back out of the matrix loc, rot, sca = mat_out.decompose() print(loc, rot, sca) # it can also be useful to access components of a matrix directly mat = mathutils.Matrix() mat[0][0], mat[1][0], mat[2][0] = 0.0, 1.0, 2.0 mat[0][0:3] = 0.0, 1.0, 2.0 # each item in a matrix is a vector so vector utility functions can be used mat[0].xyz = 0.0, 1.0, 2.0